Figure 3.

The cells derived from aged mice are more prone to oxidative stress-induced chromosomal instability. A. Bone marrow cells were freshly harvested from 2-month-old or 24-month-old mice. Bone marrow cells were cultured under normoxic conditions for one day. Karyotypic analyses for non-megakaryocytes in fresh (D0) or cultured (D1) cells were performed as described in Materials and methods. At least 100 metaphase spreads were counted for each group. B. Bone marrow cells were freshly harvested from 2- month-old or 24-month-old mice. The cells were cultured under normoxic conditions for one day. ROS levels in the fresh and cultured cells were measured as described in Materials and methods. The relative increase in ROS levels following the culture for each group of the cells was determined. C. Bone marrow cells freshly harvested from 2-month-old or 24-month-old mice were cultured in the presence of NAC (0.1 μM) under normoxic conditions for one day. Karyotypic analyses for non-megakaryocytes were performed. At least 100 metaphase spreads were counted for each group.