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. 2012 Mar 13;2:311. doi: 10.1038/srep00311

Figure 3. Engineer decarboxylation selectivity to increase production.

Figure 3

(a) Crystal structure of KIVD active site. (b) Sequence alignment of KIVD and Salmonella typhimurium IPDC. (c) Production level of isovalerate by different decarboxylases. (d) Production level of isocaproate by different decarboxylases. (e) Kinetic parameters of the most productive aldehyde dehydrogenases. i, WT KIVD. ii, F381L/V461AKIVD. iii, F382L/V461AKIVD. iv, M538A/V461AKIVD. v, F542L/V461AKIVD. vi, WT IPDC. vii, V465A IPDC. viii, V540A IPDC. ix, L544A IPDC.