TABLE 4.
Selected 16S ribosomal RNA V3 DGGE bands that increased in density during in vitro fermentation and their nearest relatives as determined by sequence analysis1
| Band no. | Donors (substrate)2 | Closest relatives (Genbank accession no.) | Similarity | Taxonomic group |
| % | ||||
| 1 | FF9 (scFOS, LNnT, HMO) | Uncultured Anaerovibrio sp. (FJ683186) | 100 | Firmicutes/ Clostridia |
| 2 | FF9,FF17 (all substrates) SR9 (HMO) | Collinsella aerofaciens (T) (AB011816) | 98 | Actinobacteria |
| 3 | FF17 (scFOS, PDX/GOS, LNnT) | Clostridium ramosum (EU869233) | 100 | Clostridium cluster XVIII |
| 4 | FF17 (scFOS) | Ruminococcus obeum A2–162 (FP929054) | 100 | Clostridium cluster XIVa |
| 53 | SR17 (all substrates) | Bacteroides vulgatus (AB510712) | 100 | Bacteroidetes |
| 6 | SR17 (all substrates) | Clostridium clostridioforme (HM008264)/Clostridium sp. MLG080-1(AY653233) | 100 | Clostridium cluster XIVa |
| 7 | SR17 (HMO) | Escherichia coli (X80728) | 100 | Proteobacteria |
| 8 | SR17 (HMO) | Uncultured Oscillibacter sp. (DQ326974) | 100 | Clostridium cluster IV |
1
DGGE, denaturing gradient gel electrophoresis; FF9, 9-d-old formula-fed piglets; FF17, 17-d-old formula-fed piglets; GOS, galactooligosaccharides; HMO, human milk oligosaccharides; LNnT, lacto- -neotetraose; PDX, polydextrose; scFOS, short-chain fructooligosaccharides; SR9, 9-d-old sow-reared piglets; SR17, 17 d-old sow-reared piglets.
2
Represents donor groups and substrates in which DGGE band density increased during the fermentation.
3
This band was quantitatively verified by qPCR.