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. Author manuscript; available in PMC: 2013 Mar 7.
Published in final edited form as: Neuron. 2012 Mar 8;73(5):962–977. doi: 10.1016/j.neuron.2011.12.033

Figure 4. In vitro chronic CORT treatment reduces AMPAR synaptic currents and synaptic GluR1 clusters via GR activation.

Figure 4

(A, B) Bar graphs showing the effect of different durations (A) and concentrations (B) of CORT on mEPSC. *: p<0.01, #: p<0.05, ANOVA. (C, D) Representative mEPSC traces (C) and statistic summary (D) showing the effect of CORT (100 nM, 7-day) on mEPSC amplitude and frequency in the presence of GR or MR antagonists in cultured PFC neurons (DIV28–30). Scale bars: 50pA, 1s. *: p<0.01, #: p<0.05, t test. (E) Immunostaining of total GluR1 and PSD-95 in PFC cultures treated with or without CORT (100 nM, 7-day). (F) Bar graphs showing the cluster density of synaptic GluR1 (co-localized, yellow puncta), total GluR1 (red puncta) and PSD-95 (green puncta) in response to CORT treatment. *: p<0.01, t test.