Figure 1.

Receptor binding assays on muscle, liver, and gill. Saturation binding assays revealed the presence of saturable, high-affinity binding sites on microsomal membrane and mitochondria fractions from liver, muscle, and gill tissue from adult trout. Liver membranes and mitochondria had the highest binding capacities and lowest affinities, whereas the data for muscle and gill were similar. Each data point shown represents the mean ± SEM of three replicates. Each binding assay was repeated three times. The B _max⁡ and K _D values listed in each panel are those estimated from the saturation binding curves. Scatchard plots derived from the saturation binding data are shown as insets in each panel.