Table 4.
Listed are structurally unique, mimotopic, peptides serving as diagnostic and therapeutic antigens. Respective peptide hydrophilic indices (HIs) are displayed in columns 5 and 7. Peptides used for initial diagnosis can be of maximum, unique length (column 4) or fractionated into pentameric (single epitope) equivalents (column 6). Either takes place. Each test peptide is synthesized with an amino-ADOOA-ADOOA linker. The amide group is used for covalent coupling to microplate wells, and the ADOO A-ADOOA construct, being very hydrophilic, solubilizes all peptides, especially those that are hydrophobic. The selected myelin dimer-cornerstone, peptide homologues for therapy are therapeutically promising because of their in vivo (a) net hydrophilicity for good solubility; (b) relatively small size for intravascular permeation; and (c) ability to simultaneously abrogate formation of up to 20 pathological myelin dimers by administering just 4 pentameric plus 2 hexameric peptides (bold highlighted in columns 4 and 6) in lieu of needing to employ up to 28 individual therapeutic pentamers and having to confront individual solubility issues, and so forth.
| MS-specific peptides sequences for diagnosis and therapy | ||||||
|---|---|---|---|---|---|---|
| Myelin protein | Full-length mimotopic peptides | HI | Constituent, single-epitope, pentameric equivalents | HI | ||
| PLP | m.s. | ADARM | 3.7 | ADARM | 3.7 | |
| MOG | m.s. | HSYQE | 0.7 | HSYQE | 0.7 | |
| RNVRF | 2.2 | RNVRF | 2.2 | |||
| VTLRI | −2.5 | VTLRI | −2.5 | |||
| MOG | i.c. | IENLH | −0.9 | IENLH | −0.9 | |
| NLHRTFE | 1 | NLHRT | 0.5 | |||
| LHRTF | −2.2 | |||||
| HRTFE | 2.6 | |||||
| KTGQF | 0.3 | KTGQF | 0.3 | |||
| MBP | i.c. | Isoforms 1,2 | DNEVFGEA | 4.7 | DNEVF | 2.2 |
| " | NEVFG | 2.0 | ||||
| " | EVFGE | 2.0 | ||||
| " | VFGEA | −1.5 | ||||
| Isoforms 1,2 | QDTAVT | 0.4 | QDTAV | 0.8 | ||
| " | DTAVT | 0.2 | ||||
| Isoforms 1,2 | PKNAW | −1 | PKNAW | −1 | ||
| MBP | i.c. | Isoforms 1,2 | DNTFKD | 6.3 | DNTFK | 3.3 |
| " | NTFKD | 3.3 | ||||
| Isoforms 1,2 | LQTIQE | −1 | LQTIQ | -3.6 | ||
| " | QTIQE | 1.2 | ||||
| MBP | i.c. | Isoform 1 | KDSHHPA | 3 | KDSHH | 5.3 |
| " | DSHHP | 2.3 | ||||
| " | SHHPA | −1.2 | ||||
| Isoforms 1,3 | HGRTQ | 2.3 | HGRTQ | 2.3 | ||
| MBP | i.c. | Isoforms 3 | YKDSHHPA | 2.5 | YKDSH | 2.5 |
| KDSHH | 5.3 | |||||
| DSHHP | 2.3 | |||||
| SHHPA | −1.2 | |||||
Bold: cornerstone epitopes.