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. Author manuscript; available in PMC: 2012 Mar 13.
Published in final edited form as: Chem Biol. 2011 Nov 23;18(11):1355–1361. doi: 10.1016/j.chembiol.2011.09.008

Figure 2. Induction of Hsp70 by Inducers of the KEAP1/NRF2/ARE Pathway Depends on HSF1 but Is Independent of NRF2.

Figure 2

(A) Chemical structures of DBA, HBB2, TBE-31, and STCA. Wild-type (B, D, and E), HSF1-knockout (C), NRF2-knockout (D), or KEAP1-knockout (E) MEFs (2 × 105 per well) in 6-well plates were exposed to vehicle (0.1% acetonitrile), DBA, HBB2, celastrol (CL), TBE-31, or STCA for 4 hr. The levels of Hsp70, HO-1, and NQO1 were evaluated by western blot 20 hr later. Of note, the STCA-treated sample in (B) and (C) was run on the same gel, but the lane between “TBE-31” and “STCA” was pulled out. The data are representative of three independent experiments.