Fig. 2.

Deficiency in LPS recognition complex prevents MCD diet-induced upregulation in the expression of NADPH complex and protects from lipid peroxidation. Mice of genotype control, TLR4 KO, and MD-2 KO were fed MCD or MCS diets for 8 wk. Liver thiobarbituric acid reactive substances (TBARS) levels (A) were analyzed as described in materials and methods. Expression of liver p47^phox (B), p67^phox (C), gp91^phox (D), and p22^phox (E) was quantified by quantitative PCR (qPCR) using specific primers and normalization against the housekeeping gene 18S. NADPH oxidase activity was determined by measuring NADP⁺-to-NADPH ratios as described in materials and methods (F). *P < 0.05 compared with the corresponding MCS group.