Fig. 2.

Apoptosis of the myocardium in rat with LVH. A: representative photograph of wheat germ agglutinin (WGA) staining using ×60 magnification. The outlines of myocytes are shown by WGA staining (red), and nuclei are stained with 4,6-diamidino-2-phenylindole (DAPI, blue). B and C: representative photograph of apoptotic myocyte and apoptotic nonmyocyte by double staining with terminal deoxyribonucleotide transferase-mediated dUTP nick-end labeling (TUNEL) and WGA. Arrows signify a TUNEL-positive myocyte (green, middle) and TUNEL-positive nonmyocyte (green, right) from a rat with LVH. Scale bar: 20 μm.