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. 2012 Feb 3;8(3):1143–1152. doi: 10.1021/ct200885r

Figure 1.

Figure 1

Current vs time trace showing Aβ1–42 has pore-forming activity in artificial bilayers. Aβ1–42 activity is characterized by heterogeneous conductances and exhibits channel-like steps, spikes, and bursts. Final Aβ1–42 concentration was 9 μM. The 20 min trace was held to an applied potential of −50 mV. Predominant steps on the trace have conductances of 140, 81, 91, and 127 pS. Bilayer was prepared by the painted technique from a 1:1 (w/w) lipid mixture of DOPS/POPE. Both sides of the bilayer chamber contained as electrolyte 150 mM KCl, 1 mM MgCl2, and 10 mM Hepes pH 7.4. l-Aβ was added to the cis side. The trans side of the chamber was virtual ground. This trace was subjected to low-pass Gaussian filtering set at 50 Hz.