Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 12;209(3):437–444. doi: 10.1084/jem.20111923

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Cook et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Treatment-induced hypoxia acutely sensitizes tumor cells to MRK003. (A) Representative immunofluorescence images of pimonidazole hydrochloride (Hypoxyprobe-1) staining with areas of hypoxia shown in bright green after 3 d of treatment (bars, 100 µm; n = 4 or more samples evaluated for each condition). (B) Levels of hypoxia in tumors treated with the combination treatment (n = 6) compared with vehicle (n = 4; **, P = 0.006) and gemcitabine (n = 4; P = 0.02) cohorts. Combination treatment was compared with GSI (n = 6; P = 0.07). GSI treatment was compared with vehicle (P = 0.01). (C) A panel of 10 KPC cell lines were tested to determine the GI50 values when treated with GSI under normoxia (units = µM). (D) KPC cell lines were examined for effects of GSI under normoxic and hypoxic conditions. The graph shown is representative of 10 cell lines. These experiments were performed in triplicate on three separate occasions (*, P < 0.001). (E) Human PDA cells were examined for effects of GSI under normoxic and hypoxic conditions. The graph shown is representative of the HPAF cell line. This experiment was performed in triplicate on two separate occasions (*, P < 0.001). (F) KPC cell lines were examined for the cytotoxic activity of gemcitabine under normoxic and hypoxic conditions. (G) qRT-PCR of survivin and Notch3 under hypoxia, and after incubation with MRK003. Error bars represent SEM.

HHS Vulnerability Disclosure