Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 12;209(3):565–580. doi: 10.1084/jem.20111908

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Jaeger et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — An extrinsic factor contributes to NK cell hyporeactivity in Genista mice. (A) Circulating NK cells from WT (top) or Genista mice (bottom) were stimulated for 4 h with YAC-1 target cells (right) or medium alone (left). Representative FACS histograms show NK1.1 and CD107a expression gated on NK1.1⁺ CD3⁻ cells. (B) Histograms representing the percentage of CD107a⁺ blood NK cells in WT or Genista mice upon stimulation with YAC-1 tumor cells. Data were pooled from three independent experiments. n = 3. (C) WT and β2mKO splenocytes were stained with two different doses of the fluorescent dye CFSE, mixed at a 1:1 ratio, and transferred into WT or Genista mice. The percentage of specific lysis of MHC-I–deficient cells corresponds to the ratio of the various populations before and 48 h after co-injection in the spleen. The experiment was performed twice. n = 2–3. (D and E) Splenocytes from WT (shaded bars) or Genista (open bars) mice were stimulated for 4 h with YAC-1 tumor targets or medium alone (D), or with anti-NK1.1, anti-NKp46, or isotype control mAb-coated plates (E). NK cell intracellular IFN-γ production (D and E, top) and degranulation (CD107a surface exposure; D and E, bottom) were measured by FACS on NK1.1⁺ CD3⁻ or NKp46⁺ CD3⁻ NK cells. Data were pooled from five independent experiments. n = 3–6. (F) Representative FACS profiles are shown for NK1.1, NKp46 surface expression, or isotype control mAb (IC, dashed) on WT (black) or Genista (gray) NK cells. (G) Splenic NK cells from WT (shaded bars) or Genista (open bars) mice were stimulated for 4 h with a mixture of PMA and ionomycin. Data show the frequencies of IFN-γ⁺ (left) or CD107a⁺ (right) NK cells among the total NK cell population in WT or Genista, or the amount of IFN-γ produced (MFI) per IFN-γ⁺ NK cells from WT or Genista (middle). MFI control values were calculated as the average of the IFN-γ mean fluorescence intensity (MFI) of IFN-γ⁺ NK cells from WT mice. (H) Purified WT spleen NK cells were transferred to WT (shaded bars) or Genista (open bars) hosts, as indicated. 7 d after transfer, the frequencies of IFN-γ–producing cells (left) and CD107a⁺ cells (right) among transferred WT NK cells were analyzed after stimulation with isotype control, anti-NK1.1, or anti-NKp46 mAb-coated plates. Experiments were repeated three times, with n = 2–4 transfer groups. Statistical significance was determined with a Mann-Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.