Table 3.
Virus titers in trachea and cloacal swabs at scheduled sampling date and at the day the chicken died
| Tissue and category | Strain name | Avg EID50 for tissue/swab specimen each day postinfection (EID50/ml)a |
Avg maximum virus titer (ml)b | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |||
| Trachea | |||||||||||
| S | rHP | 6.86 ± 1.04 | − | − | − | − | − | − | − | − | 6.86 ± 1.04 |
| WB (L/PB1) | 2.70 ± 1.46 | − | − | − | − | − | − | − | − | 2.70 ± 1.46** | |
| LP (W/MNS) | 4.20 ± 0.88 | 1.32 | − | − | − | − | − | − | − | 3.48 ± 1.61** | |
| M | LP (W/NS) | + | 2.64 ± 0.39 | − | − | − | − | − | − | − | 2.64 ± 0.39 |
| WB | 2.95 ± 0.61 | 2.72 ± 1.27 | 2.42 | 3.69 ± 0.93 | 3.02 | − | − | − | − | 3.11 ± 1*** | |
| LP (W/PB2) | + | 1.20 ± 1.45 | 5.53 | − | − | − | − | − | − | 2.09 ± 1.45 | |
| WB (L/M) | + | 2.23 ± 0.78 | 3.76 | 5.32 | − | − | − | − | − | 3.51 ± 1.77 | |
| LP | + | 4.52 ± 1.13 | 5.44 ± 0.45 | 3.88 ± 1.73 | 3.70 | 0.32 | − | − | − | 4.88 ± 1.00 | |
| LP (W/M) | + | 0.31 ± 0.19 | 5.02 | 4.20 | − | − | − | − | − | 3.14 ± 2.58 | |
| LP (W/NP) | + | 2.09 ± 0.90 | 4.47 | 2.87 | − | − | − | − | − | 3.94 ± 1.68 | |
| WB (L/NP) | + | 1.54 ± 0.34 | + | 1.31 ± 0.97 | + | 0.20 | + | + | 0.20 | 1.62 ± 0.40*** | |
| LP (W/PB1) | + | 0.22 ± 0.05 | + | 3.52 ± 0.75 | + | 0.26 | + | + | 0.20 | 3.52 ± 0.75 | |
| L | WB (L/PB2) | + | 1.03 ± 0.58 | + | 2.43 ± 2.12 | + | 2.72 ± 1.95 | + | 1.02 | 0.20 | 3.34 ± 2.26 |
| WB (L/NS) | + | 0.51 ± 0.36 | + | 1.02 ± 0.62 | + | 0.20 ± 0 | + | + | 0.20 ± 0 | 1.08 ± 0.57 | |
| WB (L/MNS) | + | 0.20 ± 0 | + | 1.34 ± 1.16 | + | 0.56 ± 0.56 | + | + | 0.20 ± 0 | 1.34 ± 1.16 | |
| LP (W/PA) | + | 0.20 ± 0 | + | 3.11 ± 0.73 | + | 0.20 ± 0 | + | + | 0.20 ± 0 | 3.11 ± 0.73 | |
| Cloaca | |||||||||||
| S | rHP | 7.12 ± 0.44 | − | − | − | − | − | − | − | − | 7.12 ± 0.44 |
| WB (L/PB1) | 2.05 ± 1.02 | − | − | − | − | − | − | − | − | 2.05 ± 1.02** | |
| LP (W/MNS) | 2.33 ± 1.42 | 0.20 | − | − | − | − | − | − | − | 1.80 ± 1.57** | |
| M | LP (W/NS) | + | 0.76 ± 0.49 | − | − | − | − | − | − | − | 0.76 ± 0.49 |
| WB | 2.60 ± 0.82 | 0.83 ± 1.44 | 2.25 | 1.92 ± 0.68 | 0.53 | − | − | − | − | 1.91 ± 1.59 | |
| LP (W/PB2) | + | 0.67 ± 0.31 | 3.53 | − | − | − | − | − | − | 1.67 ± 0.31 | |
| WB (L/M) | + | 1.77 ± 1.53 | 1.28 | 1.87 | − | − | − | − | − | 2.19 ± 1.14 | |
| LP | + | 2.11 ± 1.24 | 5.78 ± 0.95 | 2.61 ± 1.02 | 0.20 | 1.70 | − | − | − | 3.04 ± 2.09 | |
| LP (W/M) | + | 1.56 ± 1.57 | 3.07 | 0.32 | − | − | − | − | − | 1.56 ± 1.57 | |
| LP (W/NP) | + | 2.31 ± 1.26 | 2.32 | 4.32 | − | − | − | − | − | 2.99 ± 1.53 | |
| WB (L/NP) | + | 1.77 ± 0.68 | + | 2.71 ± 1.31 | + | 1.26 | + | + | 0.37 | 2.36 ± 1.27 | |
| LP (W/PB1) | + | 0.37 ± 0.33 | + | 0.78 ± 0.50 | + | 0.20 | + | + | 0.20 | 0.87 ± 0.47 | |
| L | WB (L/PB2) | + | 1.11 ± 0.91 | + | 0.32 ± 0.18 | + | 0.22 ± 0.05 | + | 0.20 | 0.20 | 1.11 ± 0.91 |
| WB (L/NS) | + | 0.54 ± 0.52 | + | 2.02 ± 1.24 | + | 0.78 ± 0.69 | + | + | 0.87 ± 1.15 | 2.02 ± 1.24 | |
| WB (L/MNS) | + | 0.31 ± 0.16 | + | 2.77 ± 0.41 | + | 1.11 ± 1.52 | + | + | 0.20 ± 0 | 2.81 ± 0.48 | |
| LP (W/PA) | + | 0.25 ± 0.21 | + | 2.00 ± 0.54 | + | 0.49 ± 0.41 | + | + | 0.23 ± 0.05 | 2.00 ± 0.54 | |
a
−, all of chickens had already died; +, specimens were not collected since no chicken died on that day.
b
**, average maximum titer was significantly lower than that of rHP (P < 0.05); ***, average maximum titer was significantly lower than that of LP (P < 0.05).