Fig 10.

Depletion of CD4 T cells subsequent to CNS T cell infiltration restores viral control and CD8 T cell effector function. JHMV-infected mice were treated with α-CD4 or control MAb at 7 and 9 days p.i. (A) Virus titers in the brains were determined by plaque assay. Titers are expressed as means ± SEM. (B) Pooled brain cells (n ≥ 4/group) isolated at day 10 p.i. were stained for CD8 and intracellular granzyme B. Mean fluorescence intensity of granzyme B staining in CD8 T cells is shown. Relative transcript levels of IFN-γ (C), perforin (D), and IL-10 (E) in purified CD8 T cells from pooled brains on day 10 p.i. were assessed by real-time PCR. Transcript levels are relative to GAPDH, and data are representative of results of two independent experiments. Trends for each transcript were similar between experiments.