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Absence of CNS CD4 T cells compromises CD8 T cell survival. JHMV-infected mice were treated with α-CD4 or control MAb at 4 and 6 days p.i. Brain cells from individual mice (n = 3 to 4) isolated at day 10 p.i. were stained for CD8, Annexin-V, and Ki-67. Percentages of Annexin-V+ (A) or Ki-67+ (B) CD8 T cells are shown. Data are representative of results of two independent experiments. Statistically significant differences were determined by unpaired t test. ***, P < 0.005.
