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Luciferase activities of MDCK cells after transfection of bicistronic construct containing 5′ and IGR IRESs. Vertical bars represent means ± standard deviations of the results of three independent experiments. RF (negative control), pRluc-Fluc; 5′ IRES, pRluc-5′ IRES-Fluc; IGR IRES, pRluc-IGR IRES-Fluc; HCV (positive control), pRluc-HCV 5′ IRES-Fluc.
