Fig 2.
Strand transfer activity of purified recombinant wild-type (IN) and R263K (INR263K) integrases. (A) Strand transfer activity expressed in relative fluorescent units (RFU) in the presence of 3 nM target DNA and variable concentrations of purified recombinant protein. (B) Strand transfer activity (RFU) in the presence of 300 nM purified recombinant protein and variable concentrations of target DNA. (C) Calculated maximum strand transfer activities for wild-type IN and INR263K with variable protein or target DNA concentration. The maximum activities in the presence of increasing concentrations of proteins (Δ Protein) were calculated by excluding the higher concentration (1,200 nM). (D) Calculated Michaelis-Menten constant (Km) for purified IN and INR263K.