Fig 3.
3′-processing activity of purified recombinant wild-type (IN) and R263K (INR263K) integrases. (A) Time-dependent 3′-processing activity expressed in relative units of time-resolved fluorescence (RFU) in the presence of 100 nM protein and 10 nM viral LTR DNA mimic (P < 0.02, paired t test). (B) 3′-processing activity (RFU) after 2 h of incubation with 10 nM target DNA and increasing concentrations of protein. (C) 3′-processing activity (RFU) after 2 h of incubation with 400 nM protein and increasing concentrations of target DNA (LTR). (D) Calculated maximum 3′-processing activities for wild-type IN and INR263K with variable protein or target DNA concentrations.