Table 3.

SPOTS analysis of point mutations introduced within the GP_477-493 epitope^a

Amino acid	14G7 epitope binding affinity
	Thr-485	Ile-486	Ala-487	Gly-488	Val-499
A	–	–	+++	–	+++
C	–	–	–	–	+
D	–	–	–	+++	+++
E	–	–	–	–	+++
F	–	–	–	–	–
G	–	–	+	+++	+
H	–	+++	+++	+++	+++
I	–	+++	–	–	+++
K	–	–	–	–	+
L	–	+	–	–	+
M	–	+++	–	+++	+++
N	–	+++	–	+++	+++
P	–	–	+++	+	+++
Q	–	–	–	–	+++
R	–	–	–	–	++
S	++	++	+++	+	+++
T	+++	+++	–	–	+++
V	–	+++	–	–	+++
W	–	–	–	–	–
Y	–	–	–	–	++

^aSummary of peptide binding results by SPOTS (17, 29). –, Mutations that abrogate binding; +, mutations that retain binding. +, ++, or +++ are qualitative assessments of SPOT intensity and binding success.