Fig 1.

Identification of MAbs recognizing BoHV-4 gL-, gH-, gH/gL-, or gB-dependent epitopes. 293T cells were transfected with the gB or gH extracellular domains or the entire gL fused to a GPI membrane anchor, resulting in gB-GPI, gH-GPI, and gL-GPI, respectively. To reconstitute epitopes depending on the gH-gL heterodimer, we cotransfected the cells with plasmids encoding gH-GPI and gL-GPI. Forty-eight hours after transfection, the cells were fixed and stained with the different MAbs as indicated. Nuclei were counterstained with DAPI.