Fig 5.

DEX-inducible cellular transcription factors stimulate specific viral promoters. Mouse neuroblastoma cells (Neuro-2A) were cotransfected with 1 μg of a CAT reporter plasmid with the IETu1 promoter (IETu1Δ1024), the bICP0 E promoter (EP-638), or the gC L promoter and 1 μg of pcDNA3.1 empty vector or 1 μg of a plasmid expressing the designated DEX-inducible transcription factor. At 48 h after transfection, the CAT reporter activity was measured. The numbers represent the fold induction divided by the empty vector control. The results are the average of three independent experiments.