Fig 5.

Impact of UNG2 depletion on virus replication and reverse transcription. (A) Virus replication in UNG2-depleted cells. Equivalent amounts of wt replication-competent viruses were produced in 293T cells and then used for infection of shLuc-transduced (plain line) or shUNG2-transduced (dashed line) HeLa-CD4 cells. Aliquots of cell culture supernatant were collected 2, 4, 6, and 8 days after infection for CAp24 quantification. (B) Quantification of viral DNA. Wild-type replication-competent viruses were produced in shRNA-transduced 293T cells as indicated and then used for infection of HeLa-CD4 cells. Viruses were normalized for CAp24 before infection. At 7 h after infection, cell samples were collected and subjected to DNA purification, and the total viral DNA was quantified via quantitative PCR using specific primers for gag. Values are the means of three independent experiments. Error bars represent one SD from the mean. Statistical significance was determined by using the Student t test (n.s., P > 0.05; *, P < 0.05; **, P < 0.01).