Fig 4.

(A) Venus DNA copy number per A549 cell equivalent (c.e.) after high-MOI infection with LV reporter virus. Venus copy numbers were calculated from a standard curve of Venus-containing plasmid. (B) High-magnification microscopy after infection of A549 cells with Venus-A4L virus. DMSO- or CMLDBU6128-treated cells are shown at 1, 8, and 24 h postinfection. Cells were fixed and stained with DAPI before visualization of viral DNA factories (DAPI) and Venus-A4L (yellow fluorescent protein [YFP]). Images were taken using a 63× objective. Arrowheads point to representative perinuclear viral factories.