Fig 6.
Influence of H5 HA MBS on cytokine responses in ferrets. Groups of ferrets (n = 3/group) were infected i.n. with 106 TCID50 of either bH5N1 (gray squares), rH5N1 (black squares), or ΔH5N1 virus (white squares). mRNA levels for various cytokines and immune-related genes from PBMCs isolated on day 3 p.i. were measured by qPCR. The average of 4 CT values for each gene was normalized relative to that of GAPDH, a housekeeping gene, and fold differences were calculated compared to preinfection levels of the same animals. Horizontal bars represent the median fold change difference for each group.