Table 1.
The influence of the H5 HA MBS on the replication of the H5N1 virus in African green monkeysa
| Sample | Virus | Mean virus titer ± SE (log10 TCID50/ml org) in tissue samples collected on indicated day p.i.: |
||||
|---|---|---|---|---|---|---|
| Day 1 | Day 2 | Day 3 | Day 4 | Day 7 | ||
| Nasopharyngeal swab | H5N1 | 3.6 ± 0.5 | 2.6 ± 0.5 | 1.7 ± 0.7 | 1.6 ± 0.5 | 2.4 ± 0.9 |
| ΔH5N1 | 2.5 ± 0.8 | 1.8 ± 0.6 | 0.8 ± 0.1 | 1.4 ± 0.8 | 0.8 ± 0.3 | |
| Nasal turbinates | H5N1 | ND | 2.3 ± 0.3 | ND | 2.0 ± 0.5 | 2.3 ± 0.8 |
| ΔH5N1 | ND | 2.0 ± 0.5 | ND | ≤1.5b | ≤1.5b | |
| Lungs | H5N1 | ND | 4.0 ± 0.6 | ND | 4.1 ± 0.3 | 2.4 ± 0.2 |
| ΔH5N1 | ND | 3.6 ± 0.5 | ND | 3.0 ± 0.4 | ≤1.5b | |
a
AGMs were infected with 2 × 106 TCID50 of either the H5N1 or ΔH5N1 virus i.n. and intratracheally. Nasal swabs were collected on the indicated days while nasal turbinates and lungs were harvested on days 2, 4, and 7 p.i. Virus titers are expressed as mean ± standard error log10 TCID50/g of tissue or per ml of swabs. ND, not determined.
b
Lower limit of detection.