Fig 6.

Immunofluorescence analysis of the viral assembly complex. HFF cells were infected with UL94HA or UL94stop virus at a multiplicity of 0.01 PFU/cell. Cells were fixed 120 h postinfection, and immunofluorescence analysis was performed with the indicated antibodies to visualize viral proteins (A) or cellular EEA1 (B). Proteins were visualized using an Alexa 546-conjugated secondary antibody (red). Nuclei are stained with Hoechst (blue). Infected cells are indicated by GFP expression from viral genome.