Fig 2.

BDV infection persists in HNPCs without altering their undifferentiated phenotype, survival, and proliferation. HNPCs were infected and analyzed at different passages postinfection (PI2, PI3, and PI10) for (A) permissivity to infection, by immunofluorescence using an anti-p40 antibody; (B) viral protein level, by Western blotting; (C) morphology, by immunofluorescence using an anti-nestin antibody; (D) nestin level, by Western blotting; and (E) proliferation. Data represent the means ± SEM from 3 independent experiments performed in quintuplicate. Statistical analysis was performed by employing the Student's t test. ns, not significant.