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. 2012 Mar;86(5):2739–2749. doi: 10.1128/JVI.06492-11

Fig 4.

Fig 4

Antibodies that target the region of E2 encompassing aa 434 to 446 do not compete with antibodies that target the region of E2 encompassing aa 412 to 423 for E1E2 binding. (A) 1A76 peptide I-Ig (■), 3A64 peptide I-Ig (●), 1A76 peptide II-Ig (□), 3A64 peptide II-Ig (○), MAb AP33 (▲), and MAb 2/69a (△) were tested for their abilities to bind to GNA-captured E1E2 protein from strain H77c. Data are presented as the differences in optical densities between the antibody sample and a negative control in the absence of target E1E2 protein. (B to E) To test for cross-competition between antibody groups, the binding of GNA-captured E1E2 to 3A64 peptide I-Ig (B), 3A64 peptide II-Ig (C), 1A76 peptide I-Ig (D), or 1A76 peptide II-Ig (E) was determined in the presence of competing MAb AP33 (▲) or MAb 2/69a (△). Data are presented as the percent binding of an uninhibited control. The mean percentages of antibody binding obtained for the highest concentrations of test and control antibodies were compared by using a t test. P values of <0.01 are indicated by double asterisks.