Fig 4.

HIV-1 within VCCs is protected from neutralizing antibodies. (A and B) HIV-1 GG-infected macrophages were either fixed and permeabilized or left untreated and incubated at 37°C with anti-CD81 (A) or anti-TfR (B) antibody and broadly neutralizing antibodies targeting GP120 (41, 46) as described in Materials and Methods. Fluorescently labeled secondary antibodies were used to detect sites of antibody binding. (C) Confocal pictures of 25 macrophages were taken at the same intensities in order to be able to quantify and compare fluorescence measurements. The Pearson's correlation coefficient of CD81, TfR, or GP120 antibody colocalizing with HIV-1 Gag was calculated with Imaris 6.4.2 (Bitplane), and the R² values were plotted in the presented graph. A Student t test was done to assess statistical significance (GraphPad Prism 5).