Fig 4.

Most abundant MuLV splicing events detected in JY cells. TopHat-predicted rightward junctions with greater than 15 reads per junction are displayed. Junctions in green shading were validated by RT-PCR, followed by cloning and Sanger sequencing. Junction 8 and junction 6 plus junction 128 represent possible splicing events leading to expression of the env gene. Junction 225 represents an in-frame splicing event predicted to give rise to a Gag-Pol-Pro polypeptide lacking functional regions of the reverse transcriptase. The number of reads spanning validated junctions is shown in parentheses.