Fig 3.
Postexposure treatment of DOG4 RV-infected mice with TriGAS rapidly reduces the accumulation of RV N mRNA in the brain. Mice were infected i.n. with 105 FFU of DOG4 RV and either mock treated with PBS (A) or treated with 107 FFU of TriGAS (B and C) 4 h later. Four mice per group were euthanized at each indicated time point, and the number of DOG4 RV N mRNA (A and B) or TriGAS RV N mRNA (C) copies in brain tissue samples was quantified by qRT-PCR as described in Materials and Methods. The results are presented as the mean N mRNA copy numbers (±SE) per 1,000 copies of L13 mRNA. The bars indicate significant differences in copy numbers between different time points determined by one-way ANOVA and Tukey's multiple-comparison test. *** and **, P < 0.001 and P < 0.01, respectively. ND, not determined.