Table 1.

Numbers of injected cells, levels of human leukocyte reconstitution, and proportions of viremic mice in 14 NSG-BLT mouse cohorts inoculated intravaginally with HIV JR-CSF

Cohort	Total no. of cells injected per mouse	No. of CD34+ cells injected per mouse	No. of HSPC injected per mouse	Mean no. of human CD45+ cells/μl	Mean no. of human CD4+ cells/μl	No. of TCID50 inoculateda (JR-CSF)	No. of viremic mice/no. of mice in cohort (%)	No. of viremic mice with no HSPC injected/no. of mice in cohort (%)
BLT-1010	6.5 × 105	4.7 × 105	4,900	532	138	1.4 × 106	6/8 (75)	0/5 (0)
BLT-1011	5.0 × 105	4.3 × 105	8,700	2,142	257	0.8 × 106	14/15 (93)	3/5 (60)
BLT-1012b	3.3 × 105	2.7 × 105	2,500	1,785	261	1.1 × 106	14/16 (88)	2/5 (40)
BLT-1013	4.4 × 105	2.7 × 105	350	324	94	1.1 × 106	7/15 (47)	0/5 (0)
BLT-1014	2.0 × 105	1.6 × 105	1,400	520	100	1.0 × 106	5/7 (71)	1/5 (20)
BLT-1015	3.4 × 105	1.5 × 105	1,100	266	9	0.02 × 106	2/10 (20)	0/2 (0)
BLT-1016	1.5 × 105	1.3 × 105	620	308	10	0.4 × 106	3/8 (38)	0/2 (0)
BLT-1017	3.7 × 105	2.6 × 105	910	1,141	148	0.03 × 106	4/8 (50)	NDc
BLT-1018	7.5 × 105	6.0 × 105	760	1,195	43	0.02 × 106	2/9 (22)	NDc
BLT-1020	4.4 × 105	2.3 × 105	150	349	129	3.7 × 106	1/8 (13)	0/5 (0)
BLT-1021	1.8 × 105	1.6 × 105	1,900	577	317	4.5 × 106	13/14 (93)	5/5 (100)
BLT-1022	11.0 × 105	8.8 × 105	5,800	1,479	140	3.0 × 106	5/9 (56)	0/5 (0)
BLT-1023	2.9 × 105	2.4 × 105	820	747	188	0.5 × 106	3/5 (60)	0/5 (0)
BLT-1101	3.0 × 105	2.2 × 105	1,100	339	82	0.5 × 106	0/5 (0)	0/5 (0)

^aAssessed by limiting dilution assay in phytohemagglutinin-activated peripheral blood mononuclear cells with supernatant p24 detection 7 days after inoculation.

^bCCR5 Δ32^+/− cohort susceptible to HIV JR-CSF infection.

^cND, not determined.