Type I interferon sensitivities of PDA cell lines. PDA cell lines and HPDE cells were either treated with 5,000 U/ml IFN-α in SFM or mock treated with SFM only. Twenty-four hours posttreatment, the cells were infected with serial dilutions of VSV-ΔM51-GFP, and infectious foci were analyzed 16 h p.i. by fluorescence microscopy to calculate the virus titer under these conditions. Treatments and infections were performed in duplicate, and average values are shown. For HPAC cells pretreated with IFN-α, virus-driven GFP signal was delayed by 24 h p.i.