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. 2012 Mar;86(6):3073–3087. doi: 10.1128/JVI.05640-11

Fig 4.

Fig 4

Permissiveness of PDA cell lines to different viruses. PDA cell lines and HPDE cells were incubated with serial dilutions of viruses. The infectious foci of VSV-ΔM51-GFP, VSV-p1-GFP (24 h p.i.), and SeV-GFP (48 h p.i.) were analyzed by fluorescence microscopy. The infectious foci of VSV-wt, CRAd-dl1520, and CRAd-hTERT were analyzed by ICC as described in Materials and Methods. Virus permissiveness (relative yield) is expressed as the log10 of the ratio of the virus titer on the pancreatic cell line under study to the titer on a reference cell line (4T1 for VSV and SeV; HeLa for CRAds). The following titers were observed on reference cell lines: VSV-wt, 1.6 × 109 CIU/ml on 4T1; VSV-ΔM51-GFP, 3.3 × 108 CIU/ml on 4T1; VSV-p1-GFP, 3 × 107 CIU/ml on 4T1; SeV-GFP, 1.5 × 107 CIU/ml on 4T1; CRAd-hTERT, 1.5 × 107 CIU/ml on HeLa; and CRAd-dl1520, 4 × 108 CIU/ml on HeLa. A relative yield of 0 indicates that the PDA cell line and a reference cell line are equally permissive to the virus, while higher numbers indicate greater permissiveness for the PDA cell line. The area of infectious foci was analyzed using Image J software (NIH): small, area of <10 (surface area units); medium, area of 10 to 30; large, area of >30; nd, not done.