Table 1.
Nudivirus-like transcripts from female stage 1 pupae and day 1 adult M. demolitora
| Gene name | Locus | Adult mean RPKM (n = 6) | Pupal mean RPKM (n = 6) | P valueb | Putative functional role |
|---|---|---|---|---|---|
| helicase | 3891 | 29 | 0 | 1.1e−05 | DNA replication |
| integrase | 3978 | 53 | 1 | 1.9e−07 | DNA replication |
| 1667 | 48 | 0 | 6.7e−07 | DNA replication | |
| p47 | 703 | 128 | 233 | 0.03 | RNA polymerase subunit |
| lef-4 | 2025 | 36 | 14 | 0.0002 | RNA polymerase subunit |
| lef-8 | 283 | 176 | NS | RNA polymerase subunit | |
| lef-9 | 3218 | 72 | 54 | NS | RNA polymerase subunit |
| lef-5 | 539: transcripts 2, 7, 8, 9 | 159 | 231 | NS | Late gene expression |
| vlf-1 | 539: transcripts 1, 3,4, 5, 6, 10 | 144 | 98 | NS | Late gene expression, capsid protein, DNA packaging |
| HzNVorf140 (vlf-1b) | 3488 | 88 | 0 | 6.4e−08 | Nudcleocapsid protein, DNA packaging |
| 2966 | 197 | 0 | 1.9e−05 | Nudcleocapsid protein, DNA packaging | |
| 2965 | 519 | 0 | 2.2e−08 | Nudcleocapsid protein, DNA packaging | |
| 38K | 318 | 511 | 2 | 4.0e−05 | Nucleocapsid protein |
| vp91 | 4098 | 210 | 0 | 7.5e−08 | Nucleocapsid protein |
| vp39 | 4,109 | 3 | 6.2e−06 | Nucleocapsid protein | |
| p74 | 1,320 | 2 | 1.1e−07 | Per os infectivity | |
| pif-1 | 382 | 0 | 6.4e−09 | Per os infectivity | |
| pif-2 | 226 | 448 | 0 | 9.4e−09 | Per os infectivity |
| pif-3 | 767 | 150 | 0 | 9.4e−08 | Per os infectivity |
| 19 kDa | 1791 | 597 | 2 | 1.7e−07 | Per os infectivity |
| odv-e56 | 13843 | 5 | 0 | 1.2e−08 | Per os infectivity |
| 1447 | 329 | 1 | 7.3e−06 | Per os infectivity | |
| 1083 | 217 | 1 | 4.6e−07 | Per os infectivity | |
| odv-e66 | 175 | 405 | 0 | 2.0e−09 | ODV envelope protein |
| 1139 | 91 | 0 | 4.7e−13 | ||
| 2331 | 197 | 0 | 3.1e−09 | ||
| 4186 | 207 | 0 | 1.0e−08 | ||
| 2730 | 244 | 0 | 1.0e−08 | ||
| HzNVorf9-1 | 864 | 2 | 1.0e−06 | Structural protein | |
| HzNVorf9-2 | 761 | 0 | 1.7e−05 | Structural protein | |
| HzNVorf106 | 332 | 508 | 1 | 8.2e−08 | Structural protein |
| PmV hypothetical protein | 756 | 546 | 0 | 1.5e−06 | Structural protein |
| Structural protein | |||||
| ac92 | 5801 | 95 | 0 | 6.9e−08 | Sulfhydryl oxidase |
| 2756 | 162 | 0 | 1.30E−08 | ||
| HzNVorf64 | 1,043 | 12 | 7.9e−08 | Unknown | |
| HzNVorf94 | 318 | 511 | 2 | 4.0e−05 | Unknown |
| HzNVorf128 | 1167 | 107 | 85 | NS | Unknown |
Expression levels in pupae and adults are indicated by RPKM values (see Materials and Methods).
NS, not significant.