Table 3.
M. demolitor transcripts identified as highly or very highly expressed in day 1 adult ovariesa
| Locusa | Category | PFAM domains | Best nr hit | Identity | Number of loci |
|---|---|---|---|---|---|
| 1068 | Variable membrane protein | Variable membrane protein precursor, Trichomonas vaginalis G3 | 19% | 1 | |
| 5485 | ELO domain-containing protein (long chain fatty acid elongation) | ELO: GNS1/SUR4 family, 2.3e−52 | Similar to CG6921-PA, Tribolium castaneum | 63% | 1 |
| 1253 | Aggregation factor | Agrin-like isoform 1, Apis mellifera | 65% | 1 | |
| 4897 | DNA Pol B2 domain-containing protein | DNA_pol_B_2: DNA polymerase type B, organellar and viral, 3.9e−07 | DNA Pol B2 domain-containing protein, Glyptapanteles flavicoxis | 73% | 1 |
| 2979 | BTB/POZ domain-containing protein | BTB: BTB/POZ domain, 2.4e−21 | Hypothetical protein, Trichoplax adhaerens | 30% | 3 |
| 4014 | BTB: BTB/POZ domain, 2.6e−18 | Hypothetical protein, Tribolium castaneum | 29% | ||
| 7763 | BTB: BTB/POZ domain, 4e−23 | Hypothetical protein, Tribolium castaneum | 35% | ||
| 3605 | Nuclear hormone receptor ligand-binding domain-containing protein | Hormone_recep: Ligand-binding domain of nuclear hormone receptor, 4e−10 | Tailless, Apis mellifera | 53% | 1 |
| 2319 | Nuclear receptor transcription factor | zf-C4, Zinc finger, C4 type (two domains), 1.5e−14 | Nuclear receptor subfamily 2 group E member 1, Acromyrmex echinatior | 91% | 1 |
| 1232 | Transcription factor | bZIP_2: Basic region leucine zipper, 3.7e−16; bZIP_1: bZIP transcription factor, 2.6e−06 | Hypothetical protein, Apis mellifera | 33% | 1 |
| 4701 | Zinc finger domain-containing protein | zf-C2H2: Zinc finger, C2H2 type, 3.8e−15, 4 copies; zf-met: Zinc finger of C2H2 type, 2e−08 | Similar to PR-domain containing protein 13, Apis mellifera | 44% | 1 |
| 38 | Ankyrin repeat protein | Ank: Ankyrin repeat, 5.7e−48, 4 copies | Hypothetical protein, Cotesia congregata | 34% | |
| 212 | Ank_2: Ankyrin repeats (3 copies), 3.2e−17, 2 copies | Hypothetical protein, “Candidatus Amoebophilus asiaticus” 5a2 | 24% | ||
| 3477 | Ank_2: Ankyrin repeats (3 copies), 2.3e−23, 2 copies | Hypothetical protein, “Candidatus Amoebophilus asiaticus” 5a2 | 34% | ||
| 4062 | Ank: Ankyrin repeat, 1.5e−14 | Ankyrin repeat protein, Trichomonas vaginalis | |||
| 1302 | Ankyrin repeat protein, Wolbachia-like | Ank_2: Ankyrin repeats (3 copies), 7.2e−33, 3 copies | Ankyrin repeat protein, Wolbachia endosymbiont wVitA of Nasonia vitripennis phage WOVitA1 | 26% | |
| 785 | Retroviral aspartyl protease domain-containing protein | RVP: Retroviral aspartyl protease, 3.3e−08 | Hypothetical protein, Tribolium castaneum | 29% | 1 |
| 1134 | Putative metalloprotease | Peptidase_M13: Peptidase family M13, 2.4e−58 | Neprilysin 2, Apis mellifera | 71% | 4 |
| 1422 | Peptidase_M13_N: Peptidase family M13, 2.8e−09 | Similar to zinc metalloprotease, Nasonia vitripennis | 68% | ||
| 1485 | Peptidase_M13_N: Peptidase family M13, 3.9e−17 | Similar to zinc metalloprotease, Nasonia vitripennis | 62% | ||
| 2862 | PFAM Peptidase_M13_N, Peptidase family M13, 1.1e−07 | Membrane metalloendopeptidase-like 1, Harpegnathos saltator | 57% | ||
| 4662 | Putative uridine phosphorylase | Uridine phosphorylase 1-like, Apis mellifera | 52% | 1 | |
| 493 | Sodium/calcium exchanger | Sodium/calcium exchanger 3, Camponotus floridanus | 86% | 2 | |
| 682 | Sodium/calcium exchanger 3, Camponotus floridanus | 88% | |||
| 4183 | Sorbitol dehydrogenase | ADH_zinc_N: Zinc-binding dehydrogenase, 1.7e−21; ADH_N: Alcohol dehydrogenase GroES-like domain, 5e−20 | Sorbitol dehydrogenase, Harpegnathos saltator | 74% | 1 |
| 1017 | Transposase domain-containing protein | Transp_Tc5_C: Tc5 transposase C-terminal domain, 4.9e−07; DDE_1: DDE superfamily endonuclease, 2.3e−06; HTH_Tnp_Tc5: Tc5 transposase DNA-binding domain, 4.2e−06 | Hypothetical protein, Solenopsis invicta | 27% | 1 |
| 1337 | Transposase domain-containing protein | DDE_1: DDE superfamily endonuclease, 1.8e−14; HTH_Tnp_Tc5: Tc5 transposase DNA-binding domain, 1.1e−05; Transp_Tc5 _C: Tc5 transposase C-terminal domain, 5.6e−05 | Hypothetical protein, Solenopsis invicta | 28% | 1 |
| Not listed | Hypothetical protein | 94 | |||
| Not listed | Conserved hypothetical protein | 30 |
a
All loci listed were differentially expressed in pupae and adult ovaries (P < 3.3 × 10−6). All loci also met the criteria of having (i) RPKM values that were >50-fold higher in adults than pupae and (ii) RPKM values that were >50 in adults (see Results).