Table 5.

M. demolitor transcripts from ovaries that are homologous to genes from D. melanogaster with essential roles in locus-specific amplification^a

Drosophila amplification gene	M. demolitor ortholog	Adult mean RPKM (n = 6)	Pupal mean RPKM (n = 6)	P value	Function in gene amplification
ORC1	1371	24	11	9.1e−06	Binds origin DNA
ORC2	5864	24	9	2.5e−05	Binds origin DNA
Cyclin E	2123	72	37	0.0002	Required for S phase of endocycle
Chiffon (Dbf4)	1535	153	86	0.004	ORCb binding
Mus101	6591	32	16	0.0003	Replication, repair
E2F1	1016	89	66	NS	S-phase transcription factor, ORC binding
E2F2					S-phase transcription factor
DP	4839	82	76	NS	Replication complex assembly
	3522	152	157	NS
Humpty dumpty	4200	14	6	5.4e−06	Unknown essential function, repair
Mcm6	1678	81	72	NS	Replication complex
Geminin					Negative regulator of gene amplification
Myb	6479	9	44	0.0002	Myb complex, origin binding
Mib130	2019	93	61	0.006	Myb complex, origin binding
	2603	120	72	0.006
Mib120	1644	31	29	NS	Myb complex, origin binding
Mib40	3127	248	153	0.005	Myb complex, origin binding
Caf1	1532	134	154	NS	Myb complex

^aExpression levels are indicated as in Table 1.

^bORC, origin recognition complex.