Fig 6.

Delivery of Tat by a second virus reactivates latent virus. (First panel) Populations of latently infected Jurkat cells at 62 days following infection with NL4-3-ΔEnv-EGFP (tat H13L). (Second panel) Latently infected populations of Jurkat cells were infected with replication-competent pBR-NL4-3-IRES-dsRed, to deliver additional Tat. (Third panel) Reactivation of latent virus was determined by measuring levels of EGFP-positive cells following infection with the second virus. (Fourth panel) dsRed-positive cells were gated, and levels of latent virus reactivation in this subpopulation were measured.