Table 1.
Copies of immunoprecipitated cyclin T1 mRNA following antagomiR treatmenta
| Anti-miR | No. of copies of cyclin T1 normalized to no. of copies in starting lysate |
|
|---|---|---|
| Ago2 IP | IgG IP | |
| Anti-miR-27b | 1,420 | 250 |
| Anti-miR-29b | 135 | 959 |
| Negative control | 3,175 | 846 |
a
Following Ago2 or IgG immunoprecipitation (IP), cyclin T1 mRNA was quantified by qPCR, and numbers of copies were calculated against a cyclin T1 standard curve and then normalized to the number of cyclin T1 copies in the starting lysate (data from a representative experiment are shown).