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Depletion of SCAP inhibits lipogenic gene expression and total lipid synthesis in HCMV-infected HFs. (A) mRNA levels of lipogenic enzymes were determined by RT-PCR using total RNA extracted from mock- and HCMV-infected cells that had been treated with the shSCAP- or shCTRL-expressing lentiviral vectors. (B) Total lipid synthesis was assayed in mock-infected cells (white bars) or HCMV-infected cells (gray bars) as described in Materials and Methods.
