Table 1.
Formation of 3Dpol(C) fibrils under different experimental conditions
Assay or component | Presence of fibrils |
---|---|
Full elongation assay | + |
Full elongation assay | |
With catalytically inactive 3Dpol | − |
Without 3Dpol | − |
Without template | − |
Without primer | − |
With aptamer 47tr (1–5 μM) | − |
With RNase (25 μg/ml) | − |
With proteinase K (500 μg/ml) | − |
With EDTA (10 mM) | − |
With DTT (5 mM) | + |