Fig 4.

The amino terminus of γ₁34.5 is required to inhibit IRF3 phosphorylation. (A) MEFs were infected with indicated viruses at 5 PFU/cell, and cell lysates were subjected to immunoblotting analysis with antibodies against IRF3, phosphorylated IRF3 (Ser³⁹⁶), and ICP27, respectively, at 3 h and 6 h postinfection. (B) Quantification of IRF3 phosphorylation. The protein bands shown in panel A were quantified using NIH ImageJ software. The data are presented as the relative amount of phosphorylated IRF3 normalized to the total level of IRF3 in each sample, with mock infection arbitrarily set at 1.0.