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. 2012 Feb;86(4):1922–1929. doi: 10.1128/JVI.06516-11

Fig 2.

Fig 2

Expression profile of Bcl-2 family antiapoptotic members determined by immunoblotting. (A) Mcl-1 expression in five mouse Mϕ cell lines, mouse L929 and murine embryonic fibroblasts (MEFs), and Syrian hamster BHK-21 cells. (B) Comparison of the expression of the five Bcl-2 family antiapoptotic proteins in BHK-21 and M1-D Mϕs, not the abundant Bcl-xL and Mcl-1 expression in BHK-21 cells. (C) Loss of Mcl-1 expression in BHK-21 cells grown in 0.1% FBS for 24 and 36 h. (D) Expression of Bcl-2 antiapoptotic proteins in BeAn virus-infected BHK-21 cells (MOI = 10) at the indicated times p.i.; the substantial reduction of Mcl-1 levels at 10 to 12 h p.i. is consistent with the induction of apoptosis at this time. (E) Densitometric analysis of Bcl-xL and Mcl-1 immunoblot data in infected BHK-21 cells over time p.i. normalized to β-actin.