Fig 7.

Analysis of the concentrated M-PMV stock. Virions from 30 ml of culture medium from HEK 293T cells expressing either the wild type or the ΔGPD mutant were pelleted and resuspended. Part of the viral lysate was used for immunoprecipitation using an anti-GPD antibody, and part was used for Western blot analyses. (A) The RT activities of the wild-type and ΔGPD virions were measured before and after anti-GPD antibody precipitation. (B) The presence of viral proteins was detected by Western blotting using rabbit antibodies raised against (from top to bottom) CA (Rb × CA) and GPD (Rb × GPD).