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. 2012 Feb;86(4):1971–1981. doi: 10.1128/JVI.06470-11

Fig 2.

Fig 2

Antiviral activity of Gag28-specific CTL clones generated from early-phase PBMCs from patient KI-092, infected with WT virus. Gag28-specific CTL clones were generated from early-phase PBMCs from KI-092 by stimulating them with WT peptide. The activities of 3 CTL clones (n = 3) were analyzed. (A) Cytotoxic activity toward 721.221-CD4-A2402 cells prepulsed with the WT or 3R peptide at concentrations of 1 to 1,000 nM. The cytotoxic activity was measured at an E:T ratio of 1:1. (B) Binding affinity to WT and 3R tetramers at concentrations of 1 to 100 nM. The MFI values of the T cell clones are shown. (C) Cytotoxic activity against 721.221-CD4-A2402 cells infected with NL-432gagSF2 (WT virus) or NL-432gagSF2-3R (3R virus). WT-virus-infected (49.1% of total cells were p24 Ag+) and 3R virus-infected (48.6% of total cells were p24 Ag+) cells were used as target cells. The cytotoxic activity was measured at E:T ratios of 0.5:1, 1:1, and 2:1. (D) Abilities of the clones to suppress the replication of WT or 3R viruses. The ability was tested at different E:T ratios. The error bars indicate standard deviations.