Fig 1.

Replication and virus production by MA/JFH-1 chimeras in Huh7.5.1 cells. (A) Schematic structures of JFH-1, MA, and two MA/JFH-1 chimeras (MA/JFH-1.1 and MA/JFH-1.2). The junction of JFH-1 and MA in the 5′ UTR is an AgeI site, and the junction of MA and JFH-1 in the NS2 region is a BsaBI site. A, AgeI; B, BsaBI; X, XbaI. (B to E) Chimeric HCV RNA replication in Huh7.5.1 cells. HCV core protein level in cells (B) and culture medium (C) and HCV RNA levels in medium (D) and infectivity of culture medium (E) from HCV RNA-transfected Huh7.5.1 cells are shown. Ten micrograms of HCV RNA was transfected into Huh7.5.1 cells, and cells and culture medium were harvested on days 1, 2, and 3. n.d., not determined. Assays were performed three times independently, and data are presented as means ± standard deviation. Dashed line indicates detection limit. wt, wild type.