Fig 4.

Effects of R167G on replication and virus production of MA/JFH-1.2 and JFH-1 in Huh7-25 cells. Ten micrograms of HCV RNA was transfected into Huh7-25 cells, and cells and medium were harvested on days 1, 2, and 3. HCV core protein levels in cells (A and D) and in medium (B and E) were measured, and infectivity of medium (C and F) was determined. n.d., not determined. Dashed line indicates the detection limit. (G) Intracellular specific infectivity and virus secretion efficiency of chimeric HCV RNA-transfected cells. Intracellular and extracellular infectivity of day 3 samples was determined, and specific infectivity and virus secretion rate were calculated. Assays were performed three times independently, and data are presented as means ± standard deviation. NA, not available.