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Comparisons of interferon sensitivity between JFH-1, MA/JFH-1.2/R167G and MA/N3H+N5BX-JFH1/5am. Two micrograms of HCV RNA was transfected into Huh7.5.1 cells, and interferon was added at the indicated concentrations at 4 h after transfection. HCV core protein levels in cells (A) and in medium (B) on day 3 were measured, and data are expressed as percent versus untreated cells (0 IU/ml). Assays were performed three times independently, and data are presented as means ± standard deviation.
