Table 2.

Oligonucleotide pairs used to generate vIRF constructs

vIRF	Oligonucleotide	Oligonucleotide sequence (5′–3′)a
R6	R6 for	ctt ggc agt gcggccgc ATG GCT ACC TGG CGC CCA CCT
	R6-HA rev	cgt gaattc tca agc gta gtc tgg gac gtc gta tgg gta TTC AAA GTG CCG ATA TAT TTC
R7	R7 for	ctt ggc agt gcggccgc ATG GCG GGC CGT GGA GTC GAT
	R7-HA rev	cgt gaattc tga agc gta gtc tgg gac gtc gta tgg gta CGA GCA GGC CAC CCC ATC ATC
R10	R10 for	ctt ggc agt gcggccgc ATG GCC GCT GGG GAA TCG AGA
	R10-HA rev	agc ggatcc tta agc gta gtc tgg gac gtc gta tgg gta TTC AAA GTG CCT ATA GAT TTC
R11	R11 for	ctt ggc agt gcggccgc ATG GCG GAA CGC GAT ATG GAT
	R11-HA rev	cgt gaattc tta agc gta gtc tgg gac gtc gta tgg gta CTT CCT CCC ATA CGG TGC GAG
K9	K9 for	ctt ggc agt gcggccgc ATG GAC CCA GCC AAA GA
	K9-HA rev	cgt gaattc cta agc gta gtc tgg gac gtc gta tgg gta TTG CAT GGC ATC CCA TAA

^aRestriction enzyme sites are in boldface type; gene-specific sequences are in uppercase type.