Figure 5.

Regulation of FLVCR in mouse retina and RPE cells in nongenetic models of iron overload. RNA was prepared from whole retina of 3-month-old mice infected in vivo with mouse cytomegalovirus (MCMV) and from primary mouse RPE cells infected in vitro with MCMV and used for semiquantitative RT-PCR (A) and real-time RT-PCR (B). In both cases, 18S RNA was used as the internal control. ARPE-19 cells (a human RPE cell line) and primary mouse RPE cells were treated with ferric ammonium citrate (FAC; 100 μg/mL; 72 hours) to cause iron overload in cells. RNA was then isolated from these cells and used for semiquantitative (C) and real-time (D) RT-PCR with 18S RNA as the internal control. *P < 0.05.